Discrimination of oats (Avena sativa L.) cultivars using isozyme markers

Identification of Oats (Avena sativa L.) Cultivars by Seed and Seedling Protein Electrophoresis

Soluble proteins were extracted from seed and seedlings of eleven oats cultivars to analyze the protein profile through SDS PAGE method. In seed protein electrophoresis totally 14 bands were observed, in these six bands were common to all cultivars. Based on the seed protein banding pattern two of these cultivars were differentiated easily. The unique band at Rf value 0.151 was common for all c...

Subcellular Localization of Proteases in Developing Leaves of Oats (Avena sativa L.).

The distribution and subcellular localization of the two major proteases present in oat (Avena sativa L. cv Victory) leaves was investigated. Both the acidic protease, active at pH 4.5, and the neutral protease, active at pH 7.5, are soluble enzymes; a few percent of the enzyme activity was ionically bound or loosely associated with organellar structures sedimenting at 1000g. On the average, 16...

Chiasma frequency, distribution and terminalization in hexaploid oats (Avena sativa L.)

Chiasma counting has been regarded as the most straightforward method of scoring the total number of crossing-over events in the genome. In organisms in which genetic analysis is difficult or impossible to perform, it is a good estimator of the level of genetic recombination. In this paper we evaluated the chiasma frequency and distribution at diakinesis of twelve Brazilian oat varieties (Avena...

Assessment Report on Avena Sativa L., Herba and Avena Sativa L., Fructus

Discrimination of the closely related A and D genomes of the hexaploid oat Avena sativa L.

A satellite DNA sequence, As120a, specific to the A-genome chromosomes in the hexaploid oat, Avena sativa L., was isolated by subcloning a fragment with internal tandem repeats from a plasmid, pAs120, that had been obtained from an Avena strigosa (As genome) genomic library. Southern and in situ hybridization showed that sequences with homology to sequences within pAs120 were dispersed througho...